ZFYVE16 sirna

ZFYVE16 siRNA (Human)

Cat. Num. AAA829337

• Gene Names: ZFYVE16; PPP1R69
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFYVE16; ZFYVE16 siRNA (Human); KIAA0305; Zinc finger FYVE domain-containing protein 16; Endofin; Endosome-associated FYVE domain protein; ZFYVE16 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZFYVE16 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1539

• Applicable Applications for ZFYVE16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFYVE16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFYVE16 sirna

siRNA to inhibit ZFYVE16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157426866
• NCBI GeneID: 9765
• NCBI Accession #: NP_001098721.1
• NCBI GenBank Nucleotide #: NM_001105251.2
• UniProt Accession #: Q7Z3T8; O15023; Q5H9U2; Q7LAU7; Q86T69; Q8N5L3; Q8NEK3
• Molecular Weight: 88,377 Da
• NCBI Official Full Name: zinc finger FYVE domain-containing protein 16 isoform a
• NCBI Official Synonym Full Names: zinc finger, FYVE domain containing 16
• NCBI Official Symbol: ZFYVE16
• NCBI Official Synonym Symbols: PPP1R69
• NCBI Protein Information: zinc finger FYVE domain-containing protein 16
• UniProt Protein Name: Zinc finger FYVE domain-containing protein 16
• Protein Family: Zinc finger FYVE domain-containing protein
• UniProt Gene Name: ZFYVE16
• UniProt Synonym Gene Names: KIAA0305
• UniProt Entry Name: ZFY16_HUMAN

NCBI Description

This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

endofin: May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 5q14

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; early endosome; cytoplasm

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein transporter activity; phosphatidylinositol binding

Biological Process: BMP signaling pathway; protein targeting to lysosome; vesicle organization and biogenesis; regulation of endocytosis; endosome transport; signal transduction