ZEB1 sirna

ZEB1 siRNA (Human)

Cat. Num. AAA8204035

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZEB1; ZEB1 siRNA (Human); AREB6; TCF8; Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8; ZEB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZEB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1108

• Applicable Applications for ZEB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZEB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZEB1 sirna

siRNA to inhibit ZEB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189409130
• NCBI GeneID: 6935
• NCBI Accession #: NP_001121600.1
• NCBI GenBank Nucleotide #: NM_001128128.2
• UniProt Accession #: P37275; Q12924; Q13800; Q2KJ05; Q5T968; Q5VZ84; Q8NB68; B4DJV0; B4DUW9; E9PCM7; F5H4I8
• Molecular Weight: 122,084 Da
• NCBI Official Full Name: zinc finger E-box-binding homeobox 1 isoform a
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• NCBI Protein Information: zinc finger E-box-binding homeobox 1
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB1
• UniProt Synonym Gene Names: AREB6; TCF8; TCF-8
• UniProt Entry Name: ZEB1_HUMAN

NCBI Description

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Uniprot Description

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: C2H2-type zinc finger protein; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of mesenchymal cell proliferation; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent

Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6