SDS-Page
MYL6B recombinant protein
MYL6B, 1-208aa, Human, His tag, E Coli
Gene Names
MYL6B; MLC1SA
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
MYL6B; 1-208aa; Human; His tag; E Coli; Myosin light chain 6B; MLC1SA; MYL6B recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPPKKDV PVKKPAGPSI SKPAAKPAAA GAPPAKTKAE PAVPQAPQKT QEPPVDLSKV VIEFNKDQLE EFKEAFELFD RVGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEL KSRRVDFETF LPMLQAVAKN RGQGTYEDYL EGFRVFDKEG NGKVMGAELR HVLTTLGEKM TEEEVETVLA GHEDSNGCIN YEAFLKHILS V
Sequence Length
208
Applicable Applications for MYL6B recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for MYL6B recombinant protein
MYL6B is a heavy chain regulator found in smooth muscle and non-muscle Myosin complexes. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain by Myosin light chain kinase. MYL6B does not bind calcium during contraction. It is primarily found in a hexamer consisting of four light chains and two heavy chains. It most commonly interacts with Myosin Va, an Actin based motor that can move in large steps. MYL6B is expressed in most tissues with neurons and smooth muscle tissue having the highest expression. Recombinant human MYL6B protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for MYL6B recombinant protein
References
Mitsui T., et al. (1992) J Biol Chem. 267 (23):16727-35.; Takeya K., et al. (2008) Am J Physiol Renal Physiol. 294 (6):1487-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25.2 kDa (231aa), confirmed by MALDI-TOF
NCBI Official Full Name
myosin light chain 6B
NCBI Official Synonym Full Names
myosin light chain 6B
NCBI Official Symbol
MYL6B
NCBI Official Synonym Symbols
MLC1SA
NCBI Protein Information
myosin light chain 6B
UniProt Protein Name
Myosin light chain 6B
Protein Family
UniProt Gene Name
MYL6B
UniProt Synonym Gene Names
MLC1SA; MLC1sa
NCBI Description
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Uniprot Description
Regulatory light chain of myosin. Does not bind calcium.
Research Articles on MYL6B
Similar Products
Product Notes
The MYL6B myl6b (Catalog #AAA204410) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's MYL6B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MYL6B myl6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMPPKKDV PVKKPAGPSI SKPAAKPAAA GAPPAKTKAE PAVPQAPQKT QEPPVDLSKV VIEFNKDQLE EFKEAFELFD RVGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEL KSRRVDFETF LPMLQAVAKN RGQGTYEDYL EGFRVFDKEG NGKVMGAELR HVLTTLGEKM TEEEVETVLA GHEDSNGCIN YEAFLKHILS V. It is sometimes possible for the material contained within the vial of "MYL6B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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