ZAK sirna

ZAK siRNA (Human)

Cat. Num. AAA8240248

• Gene Names: ZAK; pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZAK; ZAK siRNA (Human); MLTK; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK; ZAK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZAK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 800

• Applicable Applications for ZAK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZAK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZAK sirna

siRNA to inhibit ZAK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 82880648
• NCBI GeneID: 51776
• NCBI Accession #: NP_057737.2
• NCBI GenBank Nucleotide #: NM_016653.2
• UniProt Accession #: Q9NYL2; Q53SX1; Q580W8; Q59GY5; Q86YW8; Q9HCC4; Q9HCC5; Q9HDD2; Q9NYE9; B3KPG2
• Molecular Weight: 35,468 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase MLT isoform 1
• NCBI Official Symbol: ZAK
• NCBI Official Synonym Symbols: pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase MLT
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase MLT
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: ZAK
• UniProt Synonym Gene Names: MLTK; HCCS-4; MLK-related kinase; MRK
• UniProt Entry Name: MLTK_HUMAN

NCBI Description

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAK: a mixed-lineage kinase containing a leucine-zipper (LZ) and a sterile-alpha motif (SAM). It is reported to activate the JNK/SAPK and NF-kappaB pathways.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.25; Protein kinase, TKL; TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding

Biological Process: cell death; cell proliferation; response to radiation; activation of MAPKK activity; positive regulation of apoptosis; DNA damage checkpoint; response to stress; cytoskeleton organization and biogenesis; cell differentiation; cell cycle arrest; cell cycle checkpoint; protein amino acid phosphorylation; activation of JNK activity