ZADH2 sirna

ZADH2 siRNA (Human)

Cat. Num. AAA8220165

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZADH2; ZADH2 siRNA (Human); Zinc-binding alcohol dehydrogenase domain-containing protein 2; ZADH2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZADH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 254

• Applicable Applications for ZADH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZADH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZADH2 sirna

siRNA to inhibit ZADH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 807045864
• NCBI GeneID: 284273
• NCBI Accession #: NP_001293022.1
• NCBI GenBank Nucleotide #: NM_001306093.1
• UniProt Accession #: Q8N4Q0; A8KA15; B4DZ91
• Molecular Weight: 26,900 Da
• NCBI Official Full Name: zinc-binding alcohol dehydrogenase domain-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: zinc binding alcohol dehydrogenase domain containing 2
• NCBI Official Symbol: ZADH2
• NCBI Protein Information: zinc-binding alcohol dehydrogenase domain-containing protein 2
• UniProt Protein Name: Zinc-binding alcohol dehydrogenase domain-containing protein 2
• Protein Family: Zinc-binding alcohol dehydrogenase domain-containing protein
• UniProt Gene Name: ZADH2
• UniProt Entry Name: ZADH2_HUMAN

Uniprot Description

ZADH2: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: mitochondrion; peroxisome

Molecular Function: zinc ion binding; oxidoreductase activity