YTHDF2 sirna

YTHDF2 siRNA (Human)

Cat. Num. AAA8201307

• Gene Names: YTHDF2; CAHL; HGRG8; NY-REN-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YTHDF2; YTHDF2 siRNA (Human); HGRG8; YTH domain-containing family protein 2; CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2; YTHDF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: YTHDF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 529

• Applicable Applications for YTHDF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human YTHDF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YTHDF2 sirna

siRNA to inhibit YTHDF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 290543591
• NCBI GeneID: 51441
• NCBI Accession #: NP_001166299.1
• NCBI GenBank Nucleotide #: NM_001172828.1
• UniProt Accession #: Q9Y5A9; Q5VSZ9; Q8TDH0; Q9BUJ5; A6NKG4; A8K966; B4E1G7; D3DPM8
• Molecular Weight: 56,877 Da
• NCBI Official Full Name: YTH domain-containing family protein 2 isoform 2
• NCBI Official Synonym Full Names: YTH N(6)-methyladenosine RNA binding protein 2
• NCBI Official Symbol: YTHDF2
• NCBI Official Synonym Symbols: CAHL; HGRG8; NY-REN-2
• NCBI Protein Information: YTH domain-containing family protein 2
• UniProt Protein Name: YTH domain-containing family protein 2
• Protein Family: YTH domain-containing family protein
• UniProt Gene Name: YTHDF2
• UniProt Entry Name: YTHD2_HUMAN

NCBI Description

This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]

Uniprot Description

YTHDF2: high glucose-regulated protein 8. Two alternatively spliced isoforms have been described.

Protein type: RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 1p35

Biological Process: regulation of mRNA stability; humoral immune response

Disease: Longevity 1