XRN1 sirna

XRN1 siRNA (Human)

Cat. Num. AAA8238722

• Gene Names: XRN1; SEP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: XRN1; XRN1 siRNA (Human); 5'-3' exoribonuclease 1; Strand-exchange protein 1 homolog; XRN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: XRN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1694

• Applicable Applications for XRN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human XRN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for XRN1 sirna

siRNA to inhibit XRN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 544583596
• NCBI GeneID: 54464
• NCBI Accession #: NP_001269786.1
• NCBI GenBank Nucleotide #: NM_001282857.1
• UniProt Accession #: Q8IZH2; Q4G0S3; Q68D88; Q6AI24; Q6MZS8; Q86WS7; Q8N8U4; Q9UF39
• Molecular Weight: 53,818 Da
• NCBI Official Full Name: 5'-3' exoribonuclease 1 isoform c
• NCBI Official Synonym Full Names: 5'-3' exoribonuclease 1
• NCBI Official Symbol: XRN1
• NCBI Official Synonym Symbols: SEP1
• NCBI Protein Information: 5'-3' exoribonuclease 1
• UniProt Protein Name: 5'-3' exoribonuclease 1
• Protein Family: 5'-3' exoribonuclease
• UniProt Gene Name: XRN1
• UniProt Synonym Gene Names: SEP1
• UniProt Entry Name: XRN1_HUMAN

NCBI Description

This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

XRN1: Major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'-processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA. Plays a role in replication-dependent histone mRNA degradation. May act as a tumor suppressor protein in osteogenic sarcoma (OGS). Belongs to the 5'-3' exonuclease family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Tumor suppressor; EC 3.1.11.-; Ribonuclease

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: Golgi apparatus; intermediate filament cytoskeleton; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; DNA binding; exonuclease activity

Biological Process: rRNA catabolic process; gene expression; mRNA catabolic process, deadenylation-dependent decay