WNT7B sirna

WNT7B siRNA (Human)

Cat. Num. AAA8224356

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT7B; WNT7B siRNA (Human); Protein Wnt-7b; WNT7B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WNT7B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 349

• Applicable Applications for WNT7B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WNT7B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT7B sirna

siRNA to inhibit WNT7B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17505193
• NCBI GeneID: 7477
• NCBI Accession #: NP_478679.1
• NCBI GenBank Nucleotide #: NM_058238.2
• UniProt Accession #: P56706; Q96Q12; B8A596
• Molecular Weight: 39,327 Da
• NCBI Official Full Name: protein Wnt-7b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 7B
• NCBI Official Symbol: WNT7B
• NCBI Protein Information: protein Wnt-7b
• UniProt Protein Name: Protein Wnt-7b
• Protein Family: Protein
• UniProt Gene Name: WNT7B
• UniProt Entry Name: WNT7B_HUMAN

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein. [provided by RefSeq, Oct 2008]

Uniprot Description

WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: proteinaceous extracellular matrix; extracellular space; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding

Biological Process: oxygen homeostasis; response to glucocorticoid stimulus; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; forebrain regionalization; anatomical structure regression; activation of JNK activity; neuron differentiation; cellular metabolic process; central nervous system vasculogenesis; fibroblast proliferation; homeostatic process; positive regulation of cell proliferation; regulation of cell projection size; synapse organization and biogenesis; establishment and/or maintenance of polarity of embryonic epithelium; cell fate commitment; Wnt receptor signaling pathway; in utero embryonic development; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of smoothened signaling pathway; embryonic organ development; smooth muscle cell differentiation; neurite morphogenesis; lung development