WNT6 sirna

WNT6 siRNA (Human)

Cat. Num. AAA8231357

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT6; WNT6 siRNA (Human); Protein Wnt-6; WNT6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WNT6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 365

• Applicable Applications for WNT6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WNT6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT6 sirna

siRNA to inhibit WNT6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16507239
• NCBI GeneID: 7475
• NCBI Accession #: NP_006513.1
• NCBI GenBank Nucleotide #: NM_006522.3
• UniProt Accession #: Q9Y6F9; Q9H1J6; Q9H238
• Molecular Weight: 39,721 Da
• NCBI Official Full Name: protein Wnt-6
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 6
• NCBI Official Symbol: WNT6
• NCBI Protein Information: protein Wnt-6
• UniProt Protein Name: Protein Wnt-6
• Protein Family: Protein
• UniProt Gene Name: WNT6
• UniProt Entry Name: WNT6_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT6: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: frizzled binding

Biological Process: neuron differentiation; cell fate commitment; Wnt receptor signaling pathway; ureteric bud branching; positive regulation of transcription, DNA-dependent; axis specification; odontogenesis of dentine-containing teeth