WNT2 sirna

WNT2 siRNA (Human)

Cat. Num. AAA8228141

• Gene Names: WNT2; IRP; INT1L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT2; WNT2 siRNA (Human); INT1L1; IRP; Protein Wnt-2; Int-1-like protein 1; Int-1-related protein; WNT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WNT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for WNT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WNT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT2 sirna

siRNA to inhibit WNT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507927
• NCBI GeneID: 7472
• NCBI Accession #: NP_003382.1
• NCBI GenBank Nucleotide #: NM_003391.2
• UniProt Accession #: P09544; Q75N05; Q9UDP9; A4D0V1
• Molecular Weight: 40,418 Da
• NCBI Official Full Name: protein Wnt-2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family member 2
• NCBI Official Symbol: WNT2
• NCBI Official Synonym Symbols: IRP; INT1L1
• NCBI Protein Information: protein Wnt-2
• UniProt Protein Name: Protein Wnt-2
• UniProt Gene Name: WNT2
• UniProt Synonym Gene Names: INT1L1; IRP; IRP
• UniProt Entry Name: WNT2_HUMAN

NCBI Description

This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Extracellular matrix; Cell adhesion; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cytoplasm; plasma membrane; extracellular region

Molecular Function: protein binding; frizzled binding; cytokine activity; receptor agonist activity

Biological Process: lens development in camera-type eye; Wnt receptor signaling pathway; cell fate commitment; atrial cardiac muscle morphogenesis; Wnt receptor signaling pathway through beta-catenin; neuron differentiation; positive regulation of fibroblast proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cardiac muscle cell proliferation; lung development