WHSC1L1 sirna

WHSC1L1 siRNA (Human)

Cat. Num. AAA8224010

• Gene Names: WHSC1L1; NSD3; pp14328
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WHSC1L1; WHSC1L1 siRNA (Human); NSD3; Histone-lysine N-methyltransferase NSD3; Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1; WHSC1L1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WHSC1L1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 645

• Applicable Applications for WHSC1L1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WHSC1L1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WHSC1L1 sirna

siRNA to inhibit WHSC1L1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13699813
• NCBI GeneID: 54904
• NCBI Accession #: NP_060248.2
• NCBI GenBank Nucleotide #: NM_017778.2
• UniProt Accession #: Q9BZ95; Q1RMD3; Q3B796; Q6ZSA5; Q9BYU8; Q9BYU9; Q9H2M8; Q9H9W9; Q9NXA6; B7ZL11; D3DSX1
• Molecular Weight: 160,314 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase NSD3 isoform short
• NCBI Official Synonym Full Names: Wolf-Hirschhorn syndrome candidate 1-like 1
• NCBI Official Symbol: WHSC1L1
• NCBI Official Synonym Symbols: NSD3; pp14328
• NCBI Protein Information: histone-lysine N-methyltransferase NSD3
• UniProt Protein Name: Histone-lysine N-methyltransferase NSD3
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: WHSC1L1
• UniProt Synonym Gene Names: NSD3; WHSC1-like protein 1
• UniProt Entry Name: NSD3_HUMAN

NCBI Description

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]

Uniprot Description

WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Histone-binding; Oncoprotein; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; chromosome; nucleus

Molecular Function: protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid