WASF3 sirna

WASF3 siRNA (Human)

Cat. Num. AAA8205338

• Gene Names: WASF3; SCAR3; WAVE3; Brush-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WASF3; WASF3 siRNA (Human); KIAA0900; SCAR3; WAVE3; Wiskott-Aldrich syndrome protein family member 3; WASP family protein member 3; Protein WAVE-3; Verprolin homology domain-containing protein 3; WASF3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WASF3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 499

• Applicable Applications for WASF3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WASF3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WASF3 sirna

siRNA to inhibit WASF3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 634743292
• NCBI GeneID: 10810
• NCBI Accession #: NP_001278894.1
• NCBI GenBank Nucleotide #: NM_001291965.1
• UniProt Accession #: Q9UPY6; O94974; Q86VQ2
• Molecular Weight: 55,007 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 3 isoform 2
• NCBI Official Synonym Full Names: WAS protein family, member 3
• NCBI Official Symbol: WASF3
• NCBI Official Synonym Symbols: SCAR3; WAVE3; Brush-1
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 3
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 3
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF3
• UniProt Synonym Gene Names: KIAA0900; SCAR3; WAVE3; WASP family protein member 3
• UniProt Entry Name: WASF3_HUMAN

NCBI Description

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]

Uniprot Description

WASF3: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Binds actin and the Arp2/3 complex. Expressed in ovary and brain. Belongs to the SCAR/WAVE family.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: cytoskeleton; lamellipodium; cytoplasm

Molecular Function: actin binding

Biological Process: lamellipodium biogenesis; positive regulation of myelination; regulation of cell shape; actin filament polymerization; protein complex assembly; cytoskeleton organization and biogenesis; oligodendrocyte development