WASF1 sirna

WASF1 siRNA (Human)

Cat. Num. AAA827811

• Gene Names: WASF1; WAVE; SCAR1; WAVE1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WASF1; WASF1 siRNA (Human); KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1; WASF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WASF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 559

• Applicable Applications for WASF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WASF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WASF1 sirna

siRNA to inhibit WASF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68161500
• NCBI GeneID: 8936
• NCBI Accession #: NP_001020105.1
• NCBI GenBank Nucleotide #: NM_001024934.1
• UniProt Accession #: Q92558; Q5SZK7; E1P5F2
• Molecular Weight: 61,652 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 1
• NCBI Official Synonym Full Names: WAS protein family, member 1
• NCBI Official Symbol: WASF1
• NCBI Official Synonym Symbols: WAVE; SCAR1; WAVE1
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 1
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 1
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF1
• UniProt Synonym Gene Names: KIAA0269; SCAR1; WAVE1; WASP family protein member 1
• UniProt Entry Name: WASF1_HUMAN

NCBI Description

The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoskeleton; synapse; cell junction; actin cytoskeleton

Molecular Function: protein binding; protein complex binding; Rac GTPase binding; actin binding

Biological Process: actin filament polymerization; Rac protein signal transduction; protein complex assembly; cell motility