VPS36 sirna

VPS36 siRNA (Human)

Cat. Num. AAA8236460

• Gene Names: VPS36; EAP45; C13orf9; CGI-145
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VPS36; VPS36 siRNA (Human); C13orf9; EAP45; Vacuolar protein-sorting-associated protein 36; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36; VPS36 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VPS36 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 328

• Applicable Applications for VPS36 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VPS36 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VPS36 sirna

siRNA to inhibit VPS36 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 532164692
• NCBI GeneID: 51028
• NCBI Accession #: NP_001269098.1
• NCBI GenBank Nucleotide #: NM_001282169.1
• UniProt Accession #: Q86VN1; Q3ZCV7; Q5H9S1; Q5VXB6; Q9H8Z5; Q9Y3E3; A8K125
• Molecular Weight: 36,958 Da
• NCBI Official Full Name: vacuolar protein-sorting-associated protein 36 isoform 3
• NCBI Official Synonym Full Names: vacuolar protein sorting 36 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS36
• NCBI Official Synonym Symbols: EAP45; C13orf9; CGI-145
• NCBI Protein Information: vacuolar protein-sorting-associated protein 36
• UniProt Protein Name: Vacuolar protein-sorting-associated protein 36
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS36
• UniProt Synonym Gene Names: C13orf9; EAP45
• UniProt Entry Name: VPS36_HUMAN

NCBI Description

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]

Uniprot Description

VPS36: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3. Belongs to the VPS36 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: membrane; lysosome; late endosome; cytosol; nucleus; endosome

Molecular Function: protein binding; ubiquitin binding; phosphatidylinositol 3-phosphate binding

Biological Process: protein transport; regulation of transcription, DNA-dependent; transcription, DNA-dependent; endosome transport