Rho Family GTPase 1 Recombinant Protein | RND1 recombinant protein

Recombinant Rho Family GTPase 1 (RND1)

Cat. Num. AAA2033555

• Gene Names: RND1; ARHS; RHO6; RHOS
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Rho Family GTPase 1; Recombinant Rho Family GTPase 1 (RND1); ARHS; RHO6; RHOS; Ras Homolog Gene Family Member S; Rho-related GTP-binding protein Rho6; RND1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENYTACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETVDSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYEQGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPVRSLSKRLLHL PSRSELISST FKKEKAKSC
• Sequence Length: 232

• Applicable Applications for RND1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.4kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Cys229
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119578423
• NCBI GeneID: 27289
• Molecular Weight: 26,056 Da
• NCBI Official Full Name: Rho family GTPase 1
• NCBI Official Synonym Full Names: Rho family GTPase 1
• NCBI Official Symbol: RND1
• NCBI Official Synonym Symbols: ARHS; RHO6; RHOS
• NCBI Protein Information: rho-related GTP-binding protein Rho6
• UniProt Protein Name: Rho-related GTP-binding protein Rho6
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RND1
• UniProt Synonym Gene Names: RHO6
• UniProt Entry Name: RND1_HUMAN

NCBI Description

This gene encodes a protein that belongs to the Rho GTPase family. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. A similar protein in rat interacts with a microtubule regulator to control axon extension. [provided by RefSeq, Apr 2014]

Uniprot Description

RND1: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: adherens junction; cytoskeleton; cytosol; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding; receptor binding

Biological Process: actin filament organization; axon guidance; negative regulation of cell adhesion; neuron remodeling; small GTPase mediated signal transduction