UVSSA sirna

UVSSA siRNA (Human)

Cat. Num. AAA8208581

• Gene Names: UVSSA; UVSS3; KIAA1530
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UVSSA; UVSSA siRNA (Human); KIAA1530; UV-stimulated scaffold protein A; UVSSA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UVSSA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 709

• Applicable Applications for UVSSA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UVSSA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UVSSA sirna

siRNA to inhibit UVSSA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190358543
• NCBI GeneID: 57654
• NCBI Accession #: NP_065945.2
• NCBI GenBank Nucleotide #: NM_020894.2
• UniProt Accession #: Q2YD98; Q8WTX4; Q9P1Z8; A8K9E6; B2RU11
• Molecular Weight: 29,763 Da
• NCBI Official Full Name: UV-stimulated scaffold protein A
• NCBI Official Synonym Full Names: UV-stimulated scaffold protein A
• NCBI Official Symbol: UVSSA
• NCBI Official Synonym Symbols: UVSS3; KIAA1530
• NCBI Protein Information: UV-stimulated scaffold protein A
• UniProt Protein Name: UV-stimulated scaffold protein A
• Protein Family: UV-stimulated scaffold protein
• UniProt Gene Name: UVSSA
• UniProt Synonym Gene Names: KIAA1530
• UniProt Entry Name: UVSSA_HUMAN

NCBI Description

The protein encoded by this gene appears to be involved in ubiquitination and dephosphorylation of RNA polymerase II subunits that stall after UV irradiation. The encoded protein interacts with several members of the nucleotide excision repair complex to help repair UV-induced DNA damage. Defects in this gene can cause UV-sensitive syndrome 3. [provided by RefSeq, May 2012]

Uniprot Description

UVSSA: Factor involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II (RNA pol IIo) and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage. Defects in UVSSA are a cause of UV-sensitive syndrome type 3 (UVSS3). A rare autosomal recessive disorder characterized by photosensitivity and mild freckling but without neurological abnormalities or skin tumors. Belongs to the UVSSA family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: chromosome

Molecular Function: protein binding

Biological Process: transcription-coupled nucleotide-excision repair; protein ubiquitination; response to UV

Disease: Uv-sensitive Syndrome 3