USF1 sirna

USF1 siRNA (Human)

Cat. Num. AAA8205210

• Gene Names: USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USF1; USF1 siRNA (Human); BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1; USF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: USF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 310

• Applicable Applications for USF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human USF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USF1 sirna

siRNA to inhibit USF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 444909174
• NCBI GeneID: 7391
• NCBI Accession #: NP_001263302.1
• NCBI GenBank Nucleotide #: NM_001276373.1
• UniProt Accession #: P22415; Q5SY46; Q7Z5Y1; B2RBZ4
• Molecular Weight: 27,372 Da
• NCBI Official Full Name: upstream stimulatory factor 1 isoform 1
• NCBI Official Synonym Full Names: upstream transcription factor 1
• NCBI Official Symbol: USF1
• NCBI Official Synonym Symbols: UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• NCBI Protein Information: upstream stimulatory factor 1
• UniProt Protein Name: Upstream stimulatory factor 1
• Protein Family: Upstream stimulatory factor
• UniProt Gene Name: USF1
• UniProt Synonym Gene Names: BHLHB11; USF; bHLHb11
• UniProt Entry Name: USF1_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]

Uniprot Description

USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; enzyme binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; lipid homeostasis; glucose metabolic process; late viral mRNA transcription; glucose homeostasis; regulation of transcription from RNA polymerase II promoter; negative regulation of fibrinolysis; cellular response to insulin stimulus; response to hypoxia; regulation of transcription from RNA polymerase II promoter by glucose; regulation of transcription by carbon catabolites; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; response to UV

Disease: Hyperlipidemia, Combined, 1