UHRF2 sirna

UHRF2 siRNA (Human)

Cat. Num. AAA8226675

• Gene Names: UHRF2; NIRF; URF2; RNF107
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UHRF2; UHRF2 siRNA (Human); NIRF; RNF107; E3 ubiquitin-protein ligase UHRF2; Np95/ICBP90-like RING finger protein; Np95-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2; UHRF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UHRF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 503

• Applicable Applications for UHRF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UHRF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UHRF2 sirna

siRNA to inhibit UHRF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23312364
• NCBI GeneID: 115426
• NCBI Accession #: NP_690856.1
• NCBI GenBank Nucleotide #: NM_152896.2
• UniProt Accession #: Q96PU4; Q5VYR1; Q5VYR3; Q659C8; Q8TAG7
• Molecular Weight: 56,077 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UHRF2
• NCBI Official Synonym Full Names: ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase
• NCBI Official Symbol: UHRF2
• NCBI Official Synonym Symbols: NIRF; URF2; RNF107
• NCBI Protein Information: E3 ubiquitin-protein ligase UHRF2
• UniProt Protein Name: E3 ubiquitin-protein ligase UHRF2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: UHRF2
• UniProt Synonym Gene Names: NIRF; RNF107; Np95-like RING finger protein
• UniProt Entry Name: UHRF2_HUMAN

NCBI Description

This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012]

Uniprot Description

UHRF2: E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. Appears to contribute to tumorigenesis. Associated with various cancers. DNA copy number loss is found in multiple kinds of malignancies originating from the brain, breast, stomach, kidney, hematopoietic tissue and lung. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Cell cycle regulation; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; histone binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein autoubiquitination; regulation of cell cycle; protein ubiquitination; cell differentiation; cell cycle