TXN sirna

TXN siRNA (Human)

Cat. Num. AAA8203248

• Gene Names: TXN; TRX; TRDX; TRX1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TXN; TXN siRNA (Human); TRDX; TRX; TRX1; Thioredoxin; Trx; ATL-derived factor; ADF; Surface-associated sulphydryl protein; SASP; TXN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TXN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 85

• Applicable Applications for TXN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TXN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TXN sirna

siRNA to inhibit TXN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 349732256
• NCBI GeneID: 7295
• NCBI Accession #: NP_001231867.1
• NCBI GenBank Nucleotide #: NM_001244938.1
• UniProt Accession #: P10599; O60744; Q53X69; Q96KI3; Q9UDG5; B1ALW1
• Molecular Weight: 9,452 Da
• NCBI Official Full Name: thioredoxin isoform 2
• NCBI Official Synonym Full Names: thioredoxin
• NCBI Official Symbol: TXN
• NCBI Official Synonym Symbols: TRX; TRDX; TRX1
• NCBI Protein Information: thioredoxin
• UniProt Protein Name: Thioredoxin
• UniProt Gene Name: TXN
• UniProt Synonym Gene Names: TRDX; TRX; TRX1; Trx; ADF; SASP
• UniProt Entry Name: THIO_HUMAN

NCBI Description

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity

Biological Process: negative regulation of protein export from nucleus; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; activation of protein kinase B; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of protein kinase B signaling cascade; cell proliferation; response to reactive oxygen species; response to radiation; cell-cell signaling; cell redox homeostasis; regulation of protein import into nucleus, translocation; innate immune response; cell motility; positive regulation of DNA binding