TUFM sirna

TUFM siRNA (Human)

Cat. Num. AAA8239983

• Gene Names: TUFM; P43; EFTU; COXPD4; EF-TuMT
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TUFM; TUFM siRNA (Human); Elongation factor Tu mitochondrial; EF-Tu; P43; TUFM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TUFM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 455

• Applicable Applications for TUFM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TUFM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TUFM sirna

siRNA to inhibit TUFM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34147630
• NCBI GeneID: 7284
• NCBI Accession #: NP_003312.3
• NCBI GenBank Nucleotide #: NM_003321.4
• UniProt Accession #: P49411; O15276
• Molecular Weight: 49,542 Da
• NCBI Official Full Name: elongation factor Tu, mitochondrial
• NCBI Official Synonym Full Names: Tu translation elongation factor, mitochondrial
• NCBI Official Symbol: TUFM
• NCBI Official Synonym Symbols: P43; EFTU; COXPD4; EF-TuMT
• NCBI Protein Information: elongation factor Tu, mitochondrial
• UniProt Protein Name: Elongation factor Tu, mitochondrial
• UniProt Gene Name: TUFM
• UniProt Synonym Gene Names: EF-Tu
• UniProt Entry Name: EFTU_HUMAN

NCBI Description

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Protein type: Translation; Mitochondrial; RNA-binding; Translation elongation

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: mitochondrion; membrane

Molecular Function: GTPase activity; protein binding; GTP binding; translation elongation factor activity

Biological Process: translational elongation; mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 4