TSHB sirna

TSHB siRNA (Human)

Cat. Num. AAA8235947

• Gene Names: TSHB; TSH-B; TSH-BETA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TSHB; TSHB siRNA (Human); Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; Thyrotropin alfa; TSHB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TSHB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 93

• Applicable Applications for TSHB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TSHB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TSHB sirna

siRNA to inhibit TSHB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 115430099
• NCBI GeneID: 7252
• NCBI Accession #: NP_000540.2
• NCBI GenBank Nucleotide #: NM_000549.4
• UniProt Accession #: P01222; Q16163; B1AKP0
• Molecular Weight: 10,624 Da
• NCBI Official Full Name: thyrotropin subunit beta isoform 1
• NCBI Official Synonym Full Names: thyroid stimulating hormone, beta
• NCBI Official Symbol: TSHB
• NCBI Official Synonym Symbols: TSH-B; TSH-BETA
• NCBI Protein Information: thyrotropin subunit beta
• UniProt Protein Name: Thyrotropin subunit beta
• Protein Family: Thyrotropin
• UniProt Gene Name: TSHB
• UniProt Synonym Gene Names: TSH-B; TSH-beta
• UniProt Entry Name: TSHB_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TSHB: Indispensable for the control of thyroid structure and metabolism. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; cellular protein metabolic process; cell-cell signaling; response to estrogen stimulus; peptide hormone processing; response to calcium ion; response to vitamin A

Disease: Hypothyroidism, Congenital, Nongoitrous, 4