TSEN34 sirna

TSEN34 siRNA (Human)

Cat. Num. AAA8238343

• Gene Names: TSEN34; LENG5; PCH2C; SEN34; SEN34L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TSEN34; TSEN34 siRNA (Human); LENG5; SEN34; tRNA-splicing endonuclease subunit Sen34; Leukocyte receptor cluster member 5; tRNA-intron endonuclease Sen34; HsSen34; TSEN34 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TSEN34 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 310

• Applicable Applications for TSEN34 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TSEN34 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TSEN34 sirna

siRNA to inhibit TSEN34 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116875842
• NCBI GeneID: 79042
• NCBI Accession #: NP_001070914.1
• NCBI GenBank Nucleotide #: NM_001077446.3
• UniProt Accession #: Q9BSV6; Q9BVT1; Q9H6H5; A6NNB1; B0V3J1
• Molecular Weight: 33,652 Da
• NCBI Official Full Name: tRNA-splicing endonuclease subunit Sen34 isoform 1
• NCBI Official Synonym Full Names: TSEN34 tRNA splicing endonuclease subunit
• NCBI Official Symbol: TSEN34
• NCBI Official Synonym Symbols: LENG5; PCH2C; SEN34; SEN34L
• NCBI Protein Information: tRNA-splicing endonuclease subunit Sen34
• UniProt Protein Name: tRNA-splicing endonuclease subunit Sen34
• Protein Family: tRNA-splicing endonuclease
• UniProt Gene Name: TSEN34
• UniProt Synonym Gene Names: LENG5; SEN34; HsSen34
• UniProt Entry Name: SEN34_HUMAN

NCBI Description

This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Oct 2009]

Uniprot Description

TSEN34: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'- end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Defects in TSEN34 are the cause of pontocerebellar hypoplasia type 2C (PCH2C). Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings. Belongs to the tRNA-intron endonuclease family.

Protein type: Ribonuclease; EC 4.6.1.16; Nucleolus

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nucleolus; nucleus; tRNA-intron endonuclease complex

Molecular Function: tRNA-intron endonuclease activity; nucleic acid binding; lyase activity

Biological Process: tRNA-type intron splice site recognition and cleavage; mRNA processing

Disease: Pontocerebellar Hypoplasia, Type 2c