TRPV2 sirna

TRPV2 siRNA (Human)

Cat. Num. AAA8212827

• Gene Names: TRPV2; VRL; VRL1; VRL-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPV2; TRPV2 siRNA (Human); VRL; Transient receptor potential cation channel subfamily V member 2; TrpV2; Osm-9-like TRP channel 2; OTRPC2; Vanilloid receptor-like protein 1; VRL-1; TRPV2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRPV2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 764

• Applicable Applications for TRPV2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPV2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPV2 sirna

siRNA to inhibit TRPV2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20127551
• NCBI GeneID: 51393
• NCBI Accession #: NP_057197.2
• NCBI GenBank Nucleotide #: NM_016113.4
• UniProt Accession #: Q9Y5S1; Q9Y670; A6NML2; A8K0Z0
• Molecular Weight: 85,981 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 2
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily V, member 2
• NCBI Official Symbol: TRPV2
• NCBI Official Synonym Symbols: VRL; VRL1; VRL-1
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 2
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 2
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV2
• UniProt Synonym Gene Names: VRL; TrpV2; OTRPC2; VRL-1
• UniProt Entry Name: TRPV2_HUMAN

NCBI Description

This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq, Jul 2008]

Uniprot Description

TRPV2: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV2 sub-subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, cation

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cell surface; integral to plasma membrane; melanosome; plasma membrane; endomembrane system

Molecular Function: calcium channel activity; ion transmembrane transporter activity; ion channel activity; cation channel activity

Biological Process: response to temperature stimulus; response to heat; transport; sensory perception; positive regulation of axon extension; transmembrane transport