TRMU sirna

TRMU siRNA (Human)

Cat. Num. AAA8217024

• Gene Names: TRMU; MTO2; MTU1; TRMT; LCAL3; TRMT1; TRNT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRMU; TRMU siRNA (Human); MTU1; TRMT1; Mitochondrial tRNA-specific 2-thiouridylase 1; MTO2 homolog; TRMU sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRMU siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 166

• Applicable Applications for TRMU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRMU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRMU sirna

siRNA to inhibit TRMU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 544346333
• NCBI GeneID: 55687
• NCBI Accession #: NP_001269712.1
• NCBI GenBank Nucleotide #: NM_001282783.1
• UniProt Accession #: O75648; Q05C99; Q5W9C8; Q66K31; Q6ICC3; Q9NWC1; A8K3U7
• Molecular Weight: 19,154 Da
• NCBI Official Full Name: mitochondrial tRNA-specific 2-thiouridylase 1 isoform e
• NCBI Official Synonym Full Names: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
• NCBI Official Symbol: TRMU
• NCBI Official Synonym Symbols: MTO2; MTU1; TRMT; LCAL3; TRMT1; TRNT1
• NCBI Protein Information: mitochondrial tRNA-specific 2-thiouridylase 1
• UniProt Protein Name: Mitochondrial tRNA-specific 2-thiouridylase 1
• Protein Family: Mitochondrial tRNA-specific 2-thiouridylase
• UniProt Gene Name: TRMU
• UniProt Synonym Gene Names: MTU1; TRMT1
• UniProt Entry Name: MTU1_HUMAN

NCBI Description

This nuclear gene encodes a mitochondrial tRNA-modifying enzyme. The encoded protein catalyzes the 2-thiolation of uridine on the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln), resulting in the formation of 5-taurinomethyl-2-thiouridine moieties. Mutations in this gene may cause transient infantile liver failure. Polymorphisms in this gene may also influence the severity of deafness caused by mitochondrial 12S ribosomal RNA mutations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

TRMU: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2- thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base. Defects in TRMU are a cause of transient infantile liver failure (LFIT). A transient disorder of hepatic function characterized by elevated liver enzymes, jaundice, vomiting, coagulopathy, hyperbilirubinemia, increased serum lactate. Patients who survive the initial acute episode can recover, show normal development and have no recurrence. Belongs to the MnmA/TRMU family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Transferase; EC 2.8.1.-

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: mitochondrion

Molecular Function: sulfurtransferase activity; ATP binding; tRNA binding

Biological Process: tRNA processing

Disease: Deafness, Aminoglycoside-induced; Liver Failure, Infantile, Transient