TRIM21 sirna

TRIM21 siRNA (Human)

Cat. Num. AAA8208771

• Gene Names: TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM21; TRIM21 siRNA (Human); RNF81; RO52; SSA1; E3 ubiquitin-protein ligase TRIM21; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; Ro(SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21; TRIM21 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM21 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 475

• Applicable Applications for TRIM21 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM21 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM21 sirna

siRNA to inhibit TRIM21 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15208660
• NCBI GeneID: 6737
• NCBI Accession #: NP_003132.2
• NCBI GenBank Nucleotide #: NM_003141.3
• UniProt Accession #: P19474; Q5XPV5; Q96RF8
• Molecular Weight: 45,057 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM21
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Official Synonym Symbols: SSA; RO52; SSA1; RNF81; Ro/SSA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: RNF81; RO52; SSA1; SS-A
• UniProt Entry Name: RO52_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein monoubiquitination; regulation of interferon type I production; protein autoubiquitination; protein polyubiquitination; protein destabilization; cytokine and chemokine mediated signaling pathway; positive regulation of cell cycle; protein ubiquitination; cell cycle; negative regulation of viral transcription; positive regulation of virion penetration into host cell; inhibition of NF-kappaB transcription factor; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity