TREX1 sirna

TREX1 siRNA (Human)

Cat. Num. AAA8240414

• Gene Names: TREX1; CRV; AGS1; DRN3; HERNS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TREX1; TREX1 siRNA (Human); Three-prime repair exonuclease 1; 3'-5' exonuclease TREX1; DNase III; TREX1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TREX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 304

• Applicable Applications for TREX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TREX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TREX1 sirna

siRNA to inhibit TREX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 404434375
• NCBI GeneID: 11277
• NCBI Accession #: NP_009179.2
• NCBI GenBank Nucleotide #: NM_007248.3
• UniProt Accession #: Q9NSU2; Q8TEU2; Q9BPW1; Q9Y4X2; B2RCN9
• Molecular Weight: 33,212 Da
• NCBI Official Full Name: three-prime repair exonuclease 1 isoform c
• NCBI Official Synonym Full Names: three prime repair exonuclease 1
• NCBI Official Symbol: TREX1
• NCBI Official Synonym Symbols: CRV; AGS1; DRN3; HERNS
• NCBI Protein Information: three-prime repair exonuclease 1
• UniProt Protein Name: Three-prime repair exonuclease 1
• Protein Family: Three-prime repair exonuclease
• UniProt Gene Name: TREX1
• UniProt Entry Name: TREX1_HUMAN

NCBI Description

This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: EC 3.1.11.2; Deoxyribonuclease; Cell cycle regulation; DNA repair, damage; DNA-binding

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; nucleolus; nuclear envelope; cytosol; nucleus

Molecular Function: protein homodimerization activity; metal ion binding; double-stranded DNA binding; exodeoxyribonuclease III activity; 3'-5'-exodeoxyribonuclease activity; MutLalpha complex binding; adenyl deoxyribonucleotide binding; 3'-5' exonuclease activity; MutSalpha complex binding; single-stranded DNA binding

Biological Process: regulation of interferon type I production; mismatch repair; positive regulation of interferon type I production; innate immune response; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; DNA metabolic process; DNA recombination

Disease: Chilblain Lupus 1; Vasculopathy, Retinal, With Cerebral Leukodystrophy; Aicardi-goutieres Syndrome 1; Systemic Lupus Erythematosus