Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using LRP3 antibody., The lane on the left is treated with the antigen-specific peptide.)
Rabbit LRP3 Polyclonal Antibody | anti-LRP3 antibody
LRP3 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LRP3; Polyclonal Antibody; LRP3 Antibody; 105 kDa low-density lipoprotein receptor-related protein; CTD-2540B15.13; hLRp105; Low density lipoprotein receptor related protein 3; Low-density lipoprotein receptor-related protein 3; LRP-3; Lrp3; LRP3_HUMAN; anti-LRP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
LRP3 antibody detects endogenous levels of total LRP3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
770
Applicable Applications for anti-LRP3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane. Membrane > Coated Pit.
Tissue Specificity
Widely expressed. Highly expressed in skeletal muscle and ovary. Expressed at intermediate level in heart, brain, liver, pancreas, prostate and small intestine. Weakly expressed in testis, colon and leukocyte.
Predicted Cross Reactivity
Rabbit, Dog, Xenopus
Similarity
Rabbit (91%), Dog (82%), Xenopus (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using LRP3 antibody., The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using LRP3 antibody., The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9605601 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605601 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-LRP3 antibody
Function: Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. Its precise role is however unclear, since it does not bind to very low density lipoprotein (VLDL) or to LRPAP1 in vitro.
Subunit Structure: Binds GGA1 and GGA2.
Similarity: Belongs to the LDLR family.
Subunit Structure: Binds GGA1 and GGA2.
Similarity: Belongs to the LDLR family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 80 kD
Predicted: 83 kDa
Predicted: 83 kDa
NCBI Official Full Name
low-density lipoprotein receptor-related protein 3
NCBI Official Synonym Full Names
LDL receptor related protein 3
NCBI Official Symbol
LRP3
NCBI Protein Information
low-density lipoprotein receptor-related protein 3
UniProt Protein Name
Low-density lipoprotein receptor-related protein 3
Protein Family
UniProt Gene Name
LRP3
UniProt Synonym Gene Names
LRP-3; hLRp105
Uniprot Description
Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. Its precise role is however unclear, since it does not bind to very low density lipoprotein (VLDL) or to LRPAP1 in vitro.
Research Articles on LRP3
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Product Notes
The LRP3 lrp3 (Catalog #AAA9605601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRP3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LRP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the LRP3 lrp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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