TPCN2 sirna

TPCN2 siRNA (Human)

Cat. Num. AAA8211068

• Gene Names: TPCN2; TPC2; SHEP10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TPCN2; TPCN2 siRNA (Human); TPC2; Two pore calcium channel protein 2; Voltage-dependent calcium channel protein TPC2; TPCN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TPCN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 752

• Applicable Applications for TPCN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TPCN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TPCN2 sirna

siRNA to inhibit TPCN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157502181
• NCBI GeneID: 219931
• NCBI Accession #: NP_620714.2
• NCBI GenBank Nucleotide #: NM_139075.3
• UniProt Accession #: Q8NHX9; Q9NT82
• Molecular Weight: 85,243 Da
• NCBI Official Full Name: two pore calcium channel protein 2
• NCBI Official Synonym Full Names: two pore segment channel 2
• NCBI Official Symbol: TPCN2
• NCBI Official Synonym Symbols: TPC2; SHEP10
• NCBI Protein Information: two pore calcium channel protein 2
• UniProt Protein Name: Two pore calcium channel protein 2
• Protein Family: Two pore calcium channel protein
• UniProt Gene Name: TPCN2
• UniProt Synonym Gene Names: TPC2
• UniProt Entry Name: TPC2_HUMAN

NCBI Description

This gene encodes a putative cation-selective ion channel with two repeats of a six-transmembrane-domain. The protein localizes to lysosomal membranes and enables nicotinic acid adenine dinucleotide phosphate (NAADP) -induced calcium ion release from lysosome-related stores. This ubiquitously expressed gene has elevated expression in liver and kidney. Two common nonsynonymous SNPs in this gene strongly associate with blond versus brown hair pigmentation.[provided by RefSeq, Dec 2009]

Uniprot Description

TPCN2: Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.

Protein type: Transporter; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: lysosomal membrane; plasma membrane; integral to membrane; endosome membrane

Molecular Function: voltage-gated calcium channel activity; identical protein binding; protein binding; protein kinase binding

Biological Process: cellular calcium ion homeostasis; smooth muscle contraction; calcium ion transport; release of sequestered calcium ion into cytosol; transmembrane transport

Disease: Skin/hair/eye Pigmentation, Variation In, 10