TNRC6A sirna

TNRC6A siRNA (Human)

Cat. Num. AAA8220818

• Gene Names: TNRC6A; GW1; GW182; TNRC6; CAGH26
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNRC6A; TNRC6A siRNA (Human); CAGH26; KIAA1460; TNRC6; Trinucleotide repeat-containing gene 6A protein; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Protein GW1; Glycine-tryptophan protein of 182 kDa; TNRC6A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TNRC6A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1962

• Applicable Applications for TNRC6A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TNRC6A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNRC6A sirna

siRNA to inhibit TNRC6A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116805348
• NCBI GeneID: 27327
• NCBI Accession #: NP_055309.2
• NCBI GenBank Nucleotide #: NM_014494.2
• UniProt Accession #: Q8NDV7; O15408; Q658L5; Q6NVB5; Q8NEZ0; Q8TBT8; Q8TCR0; Q9NV59; Q9P268; C9JAR8
• Molecular Weight: 205,151 Da
• NCBI Official Full Name: trinucleotide repeat-containing gene 6A protein
• NCBI Official Synonym Full Names: trinucleotide repeat containing 6A
• NCBI Official Symbol: TNRC6A
• NCBI Official Synonym Symbols: GW1; GW182; TNRC6; CAGH26
• NCBI Protein Information: trinucleotide repeat-containing gene 6A protein
• UniProt Protein Name: Trinucleotide repeat-containing gene 6A protein
• Protein Family: Trinucleotide repeat-containing gene 6A protein
• UniProt Gene Name: TNRC6A
• UniProt Synonym Gene Names: CAGH26; KIAA1460; TNRC6; Protein GW1
• UniProt Entry Name: TNR6A_HUMAN

NCBI Description

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

Uniprot Description

TNRC6A: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Belongs to the GW182 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; nucleotide binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; miRNA-mediated gene silencing, negative regulation of translation; innate immune response; gene expression; miRNA-mediated gene silencing; cellular response to starvation; posttranscriptional gene silencing