TNIP1 sirna

TNIP1 siRNA (Human)

Cat. Num. AAA8223559

• Gene Names: TNIP1; VAN; NAF1; ABIN-1; nip40-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNIP1; TNIP1 siRNA (Human); KIAA0113; NAF1; TNFAIP3-interacting protein 1; A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; Nef-associated factor 1; Naf1; Nip40-1; Virion-associated nuclear shuttling protein; VAN; hVAN; TNIP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TNIP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 619

• Applicable Applications for TNIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TNIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNIP1 sirna

siRNA to inhibit TNIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 356874786
• NCBI GeneID: 10318
• NCBI Accession #: NP_001239314.1
• NCBI GenBank Nucleotide #: NM_001252385.1
• UniProt Accession #: Q15025; A4F1W8; A4F1W9; A4F1X2; A4F1X4; A4F1X5; A4F1X6; A4F1X7; A4F1X9; B7Z699; E7EPY1; E7ET96
• Molecular Weight: 62,103 Da
• NCBI Official Full Name: TNFAIP3-interacting protein 1 isoform 1
• NCBI Official Synonym Full Names: TNFAIP3 interacting protein 1
• NCBI Official Symbol: TNIP1
• NCBI Official Synonym Symbols: VAN; NAF1; ABIN-1; nip40-1
• NCBI Protein Information: TNFAIP3-interacting protein 1
• UniProt Protein Name: TNFAIP3-interacting protein 1
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: TNIP1
• UniProt Synonym Gene Names: KIAA0113; NAF1; ABIN-1; Naf1; VAN; hVAN
• UniProt Entry Name: TNIP1_HUMAN

NCBI Description

This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TNIP1: Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal; however, binding to A20/TNFAIP3 seems not to be required for this function. Inhibits NF-kappa-B activation by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcricption. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflamation; this function is mediated by association with SELPLG and dependent on phosohorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection. Interacts with TNFAIP3 and IKBKG; facilitates TNFAIP3- mediated de-ubiquitination of NEMO/IKBKG. Interacts with HIV-1 matrix protein. Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IKBKG (polyubiquitinated). Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex. Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 5q32-q33.1

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: protein binding; ubiquitin-specific protease activity; mitogen-activated protein kinase binding

Biological Process: MyD88-dependent toll-like receptor signaling pathway; leukocyte adhesion; glycoprotein biosynthetic process; translation; negative regulation of viral genome replication; positive regulation of transcription from RNA polymerase II promoter; defense response; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; positive regulation of inflammatory response