Rabbit anti-Human PKC nu Polyclonal Antibody | anti-PRKCN antibody

PKC nu (PRKD3, EPK2, PRKCN, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu) (Azide free) (HRP)

Cat. Num. AAA6334348

• Gene Names: PRKD3; EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PKC nu; Polyclonal Antibody; PKC nu (PRKD3; EPK2; PRKCN; Serine/threonine-protein kinase D3; Protein kinase C nu type; Protein kinase EPK2; nPKC-nu) (Azide free) (HRP); PRKD3; nPKC-nu; anti-PRKCN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRKCN antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the center region of human PKC nu.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PKCnu Pab in lysate of HL60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B). PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (AP7025b) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and AP7025b antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRKCN antibody

Protein kinase C (PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Product Categories/Family for anti-PRKCN antibody

Antibodies; Protein Kinases

References

Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004). Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003). Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003). Bennasser, Y., et al., Virology 303(1):174-180 (2002). Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 5031689
• NCBI GeneID: 23683
• NCBI Accession #: NP_005804.1
• NCBI GenBank Nucleotide #: NM_005813.3
• UniProt Accession #: O94806; Q53TR7; Q8NEL8; D6W587
• Molecular Weight: 68,123 Da
• NCBI Official Full Name: serine/threonine-protein kinase D3
• NCBI Official Synonym Full Names: protein kinase D3
• NCBI Official Symbol: PRKD3
• NCBI Official Synonym Symbols: EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
• NCBI Protein Information: serine/threonine-protein kinase D3; protein kinase EPK2; protein kinase C, nu; protein kinase C nu type; protein-serine/threonine kinase
• UniProt Protein Name: Serine/threonine-protein kinase D3
• UniProt Gene Name: PRKD3
• UniProt Synonym Gene Names: EPK2; PRKCN
• UniProt Entry Name: KPCD3_HUMAN