TMEM173 sirna

TMEM173 siRNA (Human)

Cat. Num. AAA8240246

• Gene Names: TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMEM173; TMEM173 siRNA (Human); ERIS; MITA; STING; Stimulator of interferon genes protein; hSTING; Endoplasmic reticulum interferon stimulator; Mediator of IRF3 activation; hMITA; Transmembrane protein 173; TMEM173 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TMEM173 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 379

• Applicable Applications for TMEM173 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMEM173 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMEM173 sirna

siRNA to inhibit TMEM173 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38093659
• NCBI GeneID: 340061
• NCBI Accession #: NP_938023.1
• NCBI GenBank Nucleotide #: NM_198282.3
• UniProt Accession #: Q86WV6; A8K3P6; B6EB35; D6RBX0; D6RE01; D6RID9
• Molecular Weight: 42,193 Da
• NCBI Official Full Name: stimulator of interferon genes protein isoform 1
• NCBI Official Synonym Full Names: transmembrane protein 173
• NCBI Official Symbol: TMEM173
• NCBI Official Synonym Symbols: ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING
• NCBI Protein Information: stimulator of interferon genes protein
• UniProt Protein Name: Stimulator of interferon genes protein
• Protein Family: Stimulator of interferon genes protein
• UniProt Gene Name: TMEM173
• UniProt Synonym Gene Names: ERIS; MITA; STING; hSTING; ERIS; hMITA
• UniProt Entry Name: STING_HUMAN

NCBI Description

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

TMEM173: Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double- stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti- viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Associates with the MHC-II complex. Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS/VISA and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Ubiquitously expressed. Belongs to the TMEM173 family.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Apoptosis; Mitochondrial; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: Golgi apparatus; mitochondrial outer membrane; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; peroxisome

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; protein kinase binding; transcription factor binding

Biological Process: regulation of interferon type I production; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; apoptosis; positive regulation of interferon type I production; positive regulation of transcription factor import into nucleus; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus; interferon-beta production

Disease: Sting-associated Vasculopathy, Infantile-onset