Western Blot (WB)
(CHML rabbit polyclonal antibody. Western Blot analysis of CHML expression in HeLa.)
Rabbit anti-Human CHML Polyclonal Antibody | anti-CHML antibody
CHML (Rab Proteins Geranylgeranyltransferase Component A 2, Choroideraemia-like Protein, Rab Escort Protein 2, REP-2, REP2) (PE)
Gene Names
CHML; REP2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CHML; Polyclonal Antibody; CHML (Rab Proteins Geranylgeranyltransferase Component A 2; Choroideraemia-like Protein; Rab Escort Protein 2; REP-2; REP2) (PE); anti-CHML antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CHML.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CHML antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human CHML, aa1-656 (NP_001812.2).
Immunogen Sequence
MADNLPTEFDVVIIGTGLPESILAAACSRSGQRVLHIDSRSYYGGNWASFSFSGLLSWLKEYQQNNDIGEESTVVWQDLIHETEEAITLRKKDETIQHTEAFCYASQDMEDNVEEIGALQKNPSLGVSNTFTEVLDSALPEESQLSYFNSDEMPAKHTQKSDTEISLEVTDVEESVEKEKYCGDKTCMHTVSDKDGDKDESKSTVEDKADEPIRNRITYSQIVKEGRRFNIDLVSKLLYSQGLLIDLLIKSDVSRYVEFKNVTRILAFREGKVEQVPCSRADVFNSKELTMVEKRMLMKFLTFCLEYEQHPDEYQAFRQCSFSEYLKTKKLTPNLQHFVLHSIAMTSESSCTTIDGLNATKNFLQCLGRFGNTPFLFPLYGQGEIPQGFCRMCAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQRINAKYFIVEDSYLSEETCSNVQYKQISRAVLITDQSILKTDLDQQTSILIVPPAEPGACAVRVTELCSSTMTCMKDTYLVHLTCSSSKTAREDLESVVKKLFTPYTETEINEEELTKPRLLWALYFNMRDSSGISRSSYNGLPSNVYVCSGPDCGLGNEHAVKQAETLFQEIFPTEEFCPPPPNPEDIIFDGDDKQPEAPGTNNVVMAKLESSEESKNLESPEKHLQN
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(CHML rabbit polyclonal antibody. Western Blot analysis of CHML expression in HeLa.)
Western Blot (WB)
(CHML rabbit polyclonal antibody. Western Blot analysis of CHML expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of CHML expression in transfected 293T cell line by CHML polyclonal antibody. Lane 1: CHML transfected lysate (74.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CHML expression in transfected 293T cell line by CHML polyclonal antibody. Lane 1: CHML transfected lysate (74.1kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-CHML antibody
Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than REP-1 in supporting prenylation of Rab3 family.
Product Categories/Family for anti-CHML antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,071 Da
NCBI Official Full Name
rab proteins geranylgeranyltransferase component A 2
NCBI Official Synonym Full Names
choroideremia-like (Rab escort protein 2)
NCBI Official Symbol
CHML
NCBI Official Synonym Symbols
REP2
NCBI Protein Information
rab proteins geranylgeranyltransferase component A 2; choroideremia-like protein; choroideraemia-like protein
UniProt Protein Name
Rab proteins geranylgeranyltransferase component A 2
Protein Family
UniProt Gene Name
CHML
UniProt Synonym Gene Names
REP2; REP-2
UniProt Entry Name
RAE2_HUMAN
NCBI Description
The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]
Uniprot Description
CHML: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than REP-1 in supporting prenylation of Rab3 family. Belongs to the Rab GDI family.
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: nucleoplasm; Rab-protein geranylgeranyltransferase complex; cytoplasm; cytosol
Molecular Function: GTPase activator activity; Rab GTPase binding; Rab-protein geranylgeranyltransferase activity
Biological Process: protein geranylgeranylation; intracellular protein transport; positive regulation of GTPase activity
Research Articles on CHML
Similar Products
Product Notes
The CHML chml (Catalog #AAA6373905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHML (Rab Proteins Geranylgeranyltransferase Component A 2, Choroideraemia-like Protein, Rab Escort Protein 2, REP-2, REP2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHML can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CHML chml for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHML, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
