TMED7 sirna

TMED7 siRNA (Human)

Cat. Num. AAA8238903

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMED7; TMED7 siRNA (Human); Transmembrane emp24 domain-containing protein 7; p24 family protein gamma-3; p24gamma3; p27; TMED7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TMED7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 188

• Applicable Applications for TMED7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMED7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMED7 sirna

siRNA to inhibit TMED7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 256985102
• NCBI GeneID: 100302736
• NCBI Accession #: NP_001157941.1
• NCBI GenBank Nucleotide #: NM_001164469.3
• UniProt Accession #: Q9Y3B3; Q8NBU8; Q8WUU6; Q96K51
• Molecular Weight: 21,233 Da
• NCBI Official Full Name: TRAM adaptor with GOLD domain isoform 2
• NCBI Official Synonym Full Names: TMED7-TICAM2 readthrough
• NCBI Official Symbol: TMED7-TICAM2
• NCBI Protein Information: TRAM adaptor with GOLD domain
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 7
• Protein Family: Transmembrane emp24 domain-containing protein
• UniProt Gene Name: TMED7
• UniProt Synonym Gene Names: p24gamma3
• UniProt Entry Name: TMED7_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring transmembrane emp24 protein transport domain containing 7 (TMED7) and toll-like receptor adaptor molecule 2 (TICAM2) genes. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. This fusion product functions to negatively regulate the adaptor MyD88-independent toll-like receptor 4 pathway. [provided by RefSeq, Nov 2010]

Uniprot Description

TMED7: Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Appears to play a role in the biosynthesis of secreted cargo including processing and post- translational modifications. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q22.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; COPII vesicle coat; endoplasmic reticulum; integral to membrane; ER-Golgi intermediate compartment; COPI vesicle coat

Biological Process: protein transport