TJP2 sirna

TJP2 siRNA (Human)

Cat. Num. AAA8204326

• Gene Names: TJP2; ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TJP2; TJP2 siRNA (Human); X104; ZO2; Tight junction protein ZO-2; Tight junction protein 2; Zona occludens protein 2; Zonula occludens protein 2; TJP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TJP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1157

• Applicable Applications for TJP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TJP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TJP2 sirna

siRNA to inhibit TJP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 282165804
• NCBI GeneID: 9414
• NCBI Accession #: NP_001163886.1
• NCBI GenBank Nucleotide #: NM_001170415.1
• UniProt Accession #: Q9UDY2; Q15883; Q5VXL0; Q5VXL1; Q8N756; Q8NI14; Q99839; A2A3H9; B7Z2R8; B7Z7T6; F5H301; F5H886
• Molecular Weight: 137,342 Da
• NCBI Official Full Name: tight junction protein ZO-2 isoform 4
• NCBI Official Synonym Full Names: tight junction protein 2
• NCBI Official Symbol: TJP2
• NCBI Official Synonym Symbols: ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11
• NCBI Protein Information: tight junction protein ZO-2
• UniProt Protein Name: Tight junction protein ZO-2
• Protein Family: Tight junction protein
• UniProt Gene Name: TJP2
• UniProt Synonym Gene Names: X104; ZO2
• UniProt Entry Name: ZO2_HUMAN

NCBI Description

This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

ZO2: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with epithelial tight junctions (TJ). May mediate dynamic changes in the composition of TJs. Plays a role in tight junctions and adherens junctions. Forms homodimers, and heterodimer with ZO-1 and ZO-3. The ZOs shuttle between the TJ and the nucleus, where they may regulate gene expression. Interacts with occluding, UBN1 and scribble. Interacts with SAFB in the nucleus. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Five isoforms of the human protein are produced by alternative promoters. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues..

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q13-q21

Cellular Component: nucleoplasm; tight junction; adherens junction; cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: protein C-terminus binding; protein binding, bridging; guanylate kinase activity; protein domain specific binding; protein binding

Biological Process: response to organic substance; apoptosis; nucleotide phosphorylation; cell structure disassembly during apoptosis; intestinal absorption

Disease: Hypercholanemia, Familial; Cholestasis, Progressive Familial Intrahepatic, 4