TAF9B sirna

TAF9B siRNA (Human)

Cat. Num. AAA8238284

• Gene Names: TAF9B; DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAF9B; TAF9B siRNA (Human); TAF9L; Transcription initiation factor TFIID subunit 9B; Neuronal cell death-related protein 7; DN-7; Transcription initiation factor TFIID subunit 9-like; Transcription-associated factor TAFII31L; TAF9B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TAF9B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 251

• Applicable Applications for TAF9B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAF9B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAF9B sirna

siRNA to inhibit TAF9B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20070280
• NCBI GeneID: 51616
• NCBI Accession #: NP_057059.2
• NCBI GenBank Nucleotide #: NM_015975.4
• UniProt Accession #: Q9HBM6; Q9Y2S3; B2RUZ9
• Molecular Weight: 27,622 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 9B
• NCBI Official Synonym Full Names: TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• NCBI Official Symbol: TAF9B
• NCBI Official Synonym Symbols: DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• NCBI Protein Information: transcription initiation factor TFIID subunit 9B
• UniProt Protein Name: Transcription initiation factor TFIID subunit 9B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF9B
• UniProt Synonym Gene Names: TAF9L; DN-7
• UniProt Entry Name: TAF9B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF9B: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. Belongs to the TAF9 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1-q21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; protein stabilization; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of apoptosis