TAF8 sirna

TAF8 siRNA (Human)

Cat. Num. AAA8236004

• Gene Names: TAF8; II; TAF; TBN; TAFII43; TAFII-43
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAF8; TAF8 siRNA (Human); TAFII43; TBN; Transcription initiation factor TFIID subunit 8; Protein taube nuss; TBP-associated factor 43 kDa; TBP-associated factor 8; Transcription initiation factor TFIID 43 kDa subunit; TAFII-43; hTAFII43; TAF8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TAF8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 338

• Applicable Applications for TAF8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAF8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAF8 sirna

siRNA to inhibit TAF8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 104485446
• NCBI GeneID: 129685
• NCBI Accession #: NP_612639.2
• NCBI GenBank Nucleotide #: NM_138572.2
• UniProt Accession #: Q7Z7C8; Q5T0K1; Q8N4R9; Q96M52
• Molecular Weight: 37,411 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 8
• NCBI Official Synonym Full Names: TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
• NCBI Official Symbol: TAF8
• NCBI Official Synonym Symbols: II; TAF; TBN; TAFII43; TAFII-43
• NCBI Protein Information: transcription initiation factor TFIID subunit 8
• UniProt Protein Name: Transcription initiation factor TFIID subunit 8
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF8
• UniProt Synonym Gene Names: TAFII43; TBN; TAFII-43; TAFII43; hTAFII43
• UniProt Entry Name: TAF8_HUMAN

NCBI Description

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; perinuclear region of cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: transcription, DNA-dependent; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; maintenance of protein localization in nucleus; cell differentiation; inner cell mass cell proliferation