TAF2 sirna

TAF2 siRNA (Human)

Cat. Num. AAA8211546

• Gene Names: TAF2; MRT40; TAF2B; CIF150; TAFII150
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAF2; TAF2 siRNA (Human); CIF150; TAF2B; Transcription initiation factor TFIID subunit 2; 150 kDa cofactor of initiator function; RNA polymerase II TBP-associated factor subunit B; TBP-associated factor 150 kDa; Transcription initiation factor TFIID 150 kDa subunit; TAF(II)150; TAFII-150; TAFII150; TAF2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TAF2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1199

• Applicable Applications for TAF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAF2 sirna

siRNA to inhibit TAF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507347
• NCBI GeneID: 6873
• NCBI Accession #: NP_003175.1
• NCBI GenBank Nucleotide #: NM_003184.3
• UniProt Accession #: Q6P1X5; O43487; O43604; O60668; Q86WW7; Q8IWK4; B2RE82
• Molecular Weight: 136,971 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 2
• NCBI Official Synonym Full Names: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
• NCBI Official Symbol: TAF2
• NCBI Official Synonym Symbols: MRT40; TAF2B; CIF150; TAFII150
• NCBI Protein Information: transcription initiation factor TFIID subunit 2
• UniProt Protein Name: Transcription initiation factor TFIID subunit 2
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF2
• UniProt Synonym Gene Names: CIF150; TAF2B; TAF(II)150; TAFII-150; TAFII150
• UniProt Entry Name: TAF2_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF2: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Belongs to the TAF2 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; G2/M transition of mitotic cell cycle; proteolysis; response to organic cyclic substance

Disease: Mental Retardation, Autosomal Recessive 40