Human TAF2 ELISA Kit | TAF2 elisa kit

Human TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit

Cat. Num. AAA2503275

• Gene Names: TAF2; MRT40; TAF2B; CIF150; TAFII150
• Reactivity: Human
• Synonyms: TAF2; Human TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit; TAF2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human TAF2. No significant cross-reactivity or interference between Human TAF2 and analogues was observed.
• Sequence Length: 1030

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAF2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TAF2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAF2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TAF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TAF2. You can calculate the concentration of TAF2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119612399
• NCBI GeneID: 6873
• UniProt Accession #: Q6P1X5; O43487; O43604; O60668; Q86WW7; Q8IWK4; B2RE82
• Molecular Weight: 136,971 Da
• NCBI Official Full Name: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa, isoform CRA_a
• NCBI Official Synonym Full Names: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
• NCBI Official Symbol: TAF2
• NCBI Official Synonym Symbols: MRT40; TAF2B; CIF150; TAFII150
• NCBI Protein Information: transcription initiation factor TFIID subunit 2; 150 kDa cofactor of initiator function; RNA polymerase II TBP-associated factor subunit B; TAF(II)150; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD; TBP-associated factor 150 kDa; cofactor of initiator function, 150kD subunit; transcription initiation factor TFIID 150 kDa subunit
• UniProt Protein Name: Transcription initiation factor TFIID subunit 2
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF2
• UniProt Synonym Gene Names: CIF150; TAF2B; TAF(II)150; TAFII-150; TAFII150
• UniProt Entry Name: TAF2_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF2: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Belongs to the TAF2 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; G2/M transition of mitotic cell cycle; proteolysis; response to organic cyclic substance

Disease: Mental Retardation, Autosomal Recessive 40