SYTL4 sirna

SYTL4 siRNA (Human)

Cat. Num. AAA827263

• Gene Names: SYTL4; SLP4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYTL4; SYTL4 siRNA (Human); Synaptotagmin-like protein 4; Exophilin-2; Granuphilin; SYTL4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SYTL4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 671

• Applicable Applications for SYTL4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SYTL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYTL4 sirna

siRNA to inhibit SYTL4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 193804862
• NCBI GeneID: 94121
• NCBI Accession #: NP_001123368.1
• NCBI GenBank Nucleotide #: NM_001129896.2
• UniProt Accession #: Q96C24; Q5H9J3; Q5JPG8; Q8N9P4; Q9H4R0; Q9H4R1
• Molecular Weight: 39,813 Da
• NCBI Official Full Name: synaptotagmin-like protein 4
• NCBI Official Synonym Full Names: synaptotagmin-like 4
• NCBI Official Symbol: SYTL4
• NCBI Official Synonym Symbols: SLP4
• NCBI Protein Information: synaptotagmin-like protein 4
• UniProt Protein Name: Synaptotagmin-like protein 4
• Protein Family: Synaptotagmin-like protein
• UniProt Gene Name: SYTL4
• UniProt Entry Name: SYTL4_HUMAN

NCBI Description

This gene encodes a member of the synaptotagmin like protein family. Members of this family are characterized by an N-terminal Rab27 binding domain and C-terminal tandem C2 domains. The encoded protein binds specific small Rab GTPases and is involved in intracellular membrane trafficking. This protein binds Rab27 and may be involved in inhibiting dense core vesicle exocytosis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Mar 2010]

Uniprot Description

SYTL4: Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: nucleoplasm; centrosome; synaptic vesicle; extrinsic to membrane; cytoplasm; plasma membrane; endosome; secretory granule

Molecular Function: zinc ion binding; transporter activity; neurexin binding; phospholipid binding; Rab GTPase binding

Biological Process: intracellular protein transport; exocytosis; positive regulation of protein secretion; negative regulation of insulin secretion; positive regulation of exocytosis