Rabbit Granuphilin Polyclonal Antibody | anti-SYTL4 antibody

Granuphilin polyclonal antibody

Cat. Num. AAA3014917

• Gene Names: SYTL4; SLP4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: Granuphilin; Polyclonal Antibody; Granuphilin polyclonal antibody; Synaptotagmin-like protein 4; Exophilin-2; SYTL4; anti-SYTL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SYTL4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human Granuphilin
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Granuphilin polyclonal antibody at 1:500 dilutionLaneA: CT26 whole cell lysate)

Related Product Information for anti-SYTL4 antibody

Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic beta-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with Insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal Insulin secretion but also inhibits high K+-induced Insulin secretion. The effect of granuphilin on Insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 193804862
• NCBI GeneID: 94121
• NCBI Accession #: NP_001123368.1
• NCBI GenBank Nucleotide #: NM_001129896.2
• UniProt Accession #: Q96C24; Q5H9J3; Q5JPG8; Q8N9P4; Q9H4R0; Q9H4R1
• Molecular Weight: 76,024 Da
• NCBI Official Full Name: synaptotagmin-like protein 4
• NCBI Official Synonym Full Names: synaptotagmin-like 4
• NCBI Official Symbol: SYTL4
• NCBI Official Synonym Symbols: SLP4
• NCBI Protein Information: synaptotagmin-like protein 4; exophilin-2; granuphilin-a
• UniProt Protein Name: Synaptotagmin-like protein 4
• Protein Family: Synaptotagmin-like protein
• UniProt Gene Name: SYTL4
• UniProt Entry Name: SYTL4_HUMAN

NCBI Description

This gene encodes a member of the synaptotagmin like protein family. Members of this family are characterized by an N-terminal Rab27 binding domain and C-terminal tandem C2 domains. The encoded protein binds specific small Rab GTPases and is involved in intracellular membrane trafficking. This protein binds Rab27 and may be involved in inhibiting dense core vesicle exocytosis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Mar 2010]

Uniprot Description

SYTL4: Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: nucleoplasm; centrosome; synaptic vesicle; extrinsic to membrane; cytoplasm; plasma membrane; endosome; secretory granule

Molecular Function: zinc ion binding; transporter activity; neurexin binding; phospholipid binding; Rab GTPase binding

Biological Process: intracellular protein transport; exocytosis; positive regulation of protein secretion; negative regulation of insulin secretion; positive regulation of exocytosis