SYTL2 sirna

SYTL2 siRNA (Human)

Cat. Num. AAA8219611

• Gene Names: SYTL2; EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYTL2; SYTL2 siRNA (Human); KIAA1597; SGA72M; SLP2; SLP2A; Synaptotagmin-like protein 2; Breast cancer-associated antigen SGA-72M; Exophilin-4; SYTL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SYTL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 934

• Applicable Applications for SYTL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SYTL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYTL2 sirna

siRNA to inhibit SYTL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 244790013
• NCBI GeneID: 54843
• NCBI Accession #: NP_001156423.1
• NCBI GenBank Nucleotide #: NM_001162951.2
• UniProt Accession #: Q9HCH5; Q2YDA7; Q6TV07; Q6ZN59; Q6ZVC5; B3KRS3; B4DJT5; B4DKW3; B4DQ26; B7SA85; B7ZLX6; B7ZLX7
• Molecular Weight: 84,651 Da
• NCBI Official Full Name: synaptotagmin-like protein 2 isoform g
• NCBI Official Synonym Full Names: synaptotagmin-like 2
• NCBI Official Symbol: SYTL2
• NCBI Official Synonym Symbols: EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151
• NCBI Protein Information: synaptotagmin-like protein 2
• UniProt Protein Name: Synaptotagmin-like protein 2
• Protein Family: Synaptotagmin-like protein
• UniProt Gene Name: SYTL2
• UniProt Synonym Gene Names: KIAA1597; SGA72M; SLP2; SLP2A
• UniProt Entry Name: SYTL2_HUMAN

NCBI Description

The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]

Uniprot Description

SYTL2: Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol- 4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon- containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca(2+) while binding to PIP2 is Ca(2+) insensitive. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rab; Motility/polarity/chemotaxis; Lipid-binding; Vesicle

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: nucleoplasm; extrinsic to plasma membrane; Golgi apparatus; membrane; cytoplasm; melanosome; plasma membrane; nucleus

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; phosphatidylserine binding; neurexin binding; Rab GTPase binding; phosphatase binding

Biological Process: vesicle-mediated transport; intracellular protein transport; exocytosis; vesicle docking during exocytosis