SULT1A3 sirna

SULT1A3 siRNA (Human)

Cat. Num. AAA8213513

• Gene Names: SULT1A3; STM; HAST; HAST3; M-PST; ST1A3; ST1A5; TL-PST; ST1A3/ST1A4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SULT1A3; SULT1A3 siRNA (Human); STM; Sulfotransferase 1A3/1A4; ST1A3/ST1A4; Aryl sulfotransferase 1A3/1A4; Catecholamine-sulfating phenol sulfotransferase; HAST3; M-PST; Monoamine-sulfating phenol sulfotransferase; Placental estrogen sulfotransferase; Sulfotransferase. monoamine-preferring; Thermolabile phenol sulfotransferase; TL-PST; SULT1A3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SULT1A3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder

• Applicable Applications for SULT1A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SULT1A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SULT1A3 sirna

siRNA to inhibit SULT1A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 1711609
• NCBI GeneID: 6818
• UniProt Accession #: P50224; P0DMM9
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
• NCBI Official Symbol: SULT1A3
• NCBI Official Synonym Symbols: STM; HAST; HAST3; M-PST; ST1A3; ST1A5; TL-PST; ST1A3/ST1A4
• NCBI Protein Information: sulfotransferase 1A3
• Protein Family: Sulfotransferase

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. This gene is the most centromeric of the four sulfotransferase genes. Read-through transcription exists between this gene and the upstream SLX1A (SLX1 structure-specific endonuclease subunit homolog A) gene that encodes a protein containing GIY-YIG domains. [provided by RefSeq, Nov 2010]

Uniprot Description

SULT1A3: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - sulfur; Transferase; EC 2.8.2.1

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; aryl sulfotransferase activity

Biological Process: steroid metabolic process; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; flavonoid metabolic process; unfolded protein response; xenobiotic metabolic process; sulfation; catecholamine metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process