STT3B sirna

STT3B siRNA (Human)

Cat. Num. AAA8229432

• Gene Names: STT3B; SIMP; CDG1X; STT3-B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: STT3B; STT3B siRNA (Human); SIMP; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; Oligosaccharyl transferase subunit STT3B; STT3-B; Source of immunodominant MHC-associated peptides homolog; STT3B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: STT3B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 826

• Applicable Applications for STT3B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human STT3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for STT3B sirna

siRNA to inhibit STT3B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30578410
• NCBI GeneID: 201595
• NCBI Accession #: NP_849193.1
• NCBI GenBank Nucleotide #: NM_178862.2
• UniProt Accession #: Q8TCJ2; Q96JZ4; Q96KY7
• Molecular Weight: 93,674 Da
• NCBI Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
• NCBI Official Synonym Full Names: STT3B, subunit of the oligosaccharyltransferase complex (catalytic)
• NCBI Official Symbol: STT3B
• NCBI Official Synonym Symbols: SIMP; CDG1X; STT3-B
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
• Protein Family: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
• UniProt Gene Name: STT3B
• UniProt Synonym Gene Names: SIMP; Oligosaccharyl transferase subunit STT3B; STT3-B
• UniProt Entry Name: STT3B_HUMAN

NCBI Description

The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014]

Uniprot Description

STT3B: Catalytic component of oligosaccharyltransferase (OST) oligosaccharyltransferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X- Ser/Thr consensus motif in nascent polypeptide chains. N- glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N- glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation. Belongs to the STT3 family.

Protein type: EC 2.4.99.18; Membrane protein, integral; Membrane protein, multi-pass; Glycan Metabolism - N-glycan biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 3p23

Cellular Component: membrane; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity

Biological Process: ER-associated protein catabolic process; co-translational protein modification; protein amino acid N-linked glycosylation via asparagine; glycoprotein catabolic process; post-translational protein modification; response to unfolded protein

Disease: Congenital Disorder Of Glycosylation, Type Ix