Rat Tyrosinase (TYR) ELISA Kit | TYR elisa kit

Rat Tyrosinase (TYR) ELISA Kit

Cat. Num. AAA2019362

• Gene Names: TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• Reactivity: Rat
• Synonyms: Tyrosinase (TYR); Rat Tyrosinase (TYR) ELISA Kit; OCAIA; OCA1A; OCA1; CMM8; Oculocutaneous Albinism IA; Monophenol monooxygenase; Tumor rejection antigen AB; TYR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tyrosinase (TYR).; No significant cross-reactivity or interference between Tyrosinase (TYR) and analogues was observed.
• Sequence Length: 529

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosinase (TYR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosinase (TYR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TYR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosinase (TYR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosinase (TYR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosinase (TYR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosinase (TYR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TYR elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4507753
• NCBI GeneID: 7299
• NCBI Accession #: NP_000363.1
• NCBI GenBank Nucleotide #: NM_000372.4
• UniProt Accession #: P14679; Q15675; Q15676; Q15680; Q8TAK4; Q9BYY0; Q9BZX1
• Molecular Weight: 42,914 Da
• NCBI Official Full Name: tyrosinase
• NCBI Official Synonym Full Names: tyrosinase
• NCBI Official Symbol: TYR
• NCBI Official Synonym Symbols: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
• NCBI Protein Information: tyrosinase
• UniProt Protein Name: Tyrosinase
• UniProt Gene Name: TYR
• UniProt Entry Name: TYRO_HUMAN

NCBI Description

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 1.14.18.1; Oxidoreductase; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; melanosome; integral to membrane; Golgi-associated vesicle

Molecular Function: protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; monophenol monooxygenase activity

Biological Process: cell proliferation; thymus development; visual perception; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process

Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3