SRY sirna

SRY siRNA (Human)

Cat. Num. AAA8200071

• Gene Names: SRY; TDF; TDY; SRXX1; SRXY1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SRY; SRY siRNA (Human); TDF; Sex-determining region Y protein; Testis-determining factor; SRY sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SRY siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 204

• Applicable Applications for SRY sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SRY gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SRY sirna

siRNA to inhibit SRY expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507225
• NCBI GeneID: 6736
• NCBI Accession #: NP_003131.1
• NCBI GenBank Nucleotide #: NM_003140.2
• UniProt Accession #: Q05066
• Molecular Weight: 23,884 Da
• NCBI Official Full Name: sex-determining region Y protein
• NCBI Official Synonym Full Names: sex determining region Y
• NCBI Official Symbol: SRY
• NCBI Official Synonym Symbols: TDF; TDY; SRXX1; SRXY1
• NCBI Protein Information: sex-determining region Y protein
• UniProt Protein Name: Sex-determining region Y protein
• Protein Family: SRY-related protein
• UniProt Gene Name: SRY
• UniProt Synonym Gene Names: TDF
• UniProt Entry Name: SRY_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

SRY: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. Defects in SRY are the cause of 46,XY sex reversal type 1 (SRXY1). A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients manifest rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, streak gonads, variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Defects in SRY are the cause of 46,XX sex reversal type 1 (SRXX1). A condition in which male gonads develop in a genetic female (female to male sex reversal). Belongs to the SRY family.

Protein type: DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Yp11.3

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; calmodulin binding; DNA binding; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; male sex determination; positive regulation of transcription, DNA-dependent; cell differentiation; sex differentiation

Disease: 46,xx Sex Reversal 1; 46,xy Sex Reversal 1