SPECC1L sirna

SPECC1L siRNA (Human)

Cat. Num. AAA8218494

• Gene Names: SPECC1L; CYTSA; GBBB2; OBLFC1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPECC1L; SPECC1L siRNA (Human); CYTSA; KIAA0376; Cytospin-A; Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like; SPECC1-like protein; SPECC1L sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPECC1L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1078

• Applicable Applications for SPECC1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPECC1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPECC1L sirna

siRNA to inhibit SPECC1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 513788264
• NCBI GeneID: 23384
• NCBI Accession #: NP_001241661.2
• NCBI GenBank Nucleotide #: NM_001254732.2
• UniProt Accession #: Q69YQ0; O15081; B7Z758; F5H1H6
• Molecular Weight: 120,182 Da
• NCBI Official Full Name: cytospin-A isoform 2
• NCBI Official Synonym Full Names: sperm antigen with calponin homology and coiled-coil domains 1-like
• NCBI Official Symbol: SPECC1L
• NCBI Official Synonym Symbols: CYTSA; GBBB2; OBLFC1
• NCBI Protein Information: cytospin-A
• UniProt Protein Name: Cytospin-A
• Protein Family: Cytospin
• UniProt Gene Name: SPECC1L
• UniProt Synonym Gene Names: CYTSA; KIAA0376; SPECC1-like protein
• UniProt Entry Name: CYTSA_HUMAN

NCBI Description

This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

SPECC1L: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Defects in SPECC1L are the cause of facial clefting oblique type 1 (OBLFC1). A rare form of facial clefting. A facial cleft is any of the fissures between the embryonic prominences that normally unite to form the face. Belongs to the cytospin-A family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: filamentous actin; cytoplasm; gap junction; microtubule organizing center; spindle

Biological Process: cell migration; negative regulation of microtubule depolymerization; cell division; negative regulation of actin filament depolymerization; actin cytoskeleton organization and biogenesis; cell cycle

Disease: Facial Clefting, Oblique, 1; Opitz Gbbb Syndrome, Type Ii