SPANXN4 sirna

SPANXN4 siRNA (Human)

Cat. Num. AAA828266

• Gene Names: SPANXN4; CT11.9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPANXN4; SPANXN4 siRNA (Human); Sperm protein associated with the nucleus on the X chromosome N4; Nuclear-associated protein SPAN-Xn4; SPANX-N4; SPANX family member N4; SPANXN4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPANXN4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 99

• Applicable Applications for SPANXN4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPANXN4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPANXN4 sirna

siRNA to inhibit SPANXN4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 57528117
• NCBI GeneID: 441525
• NCBI Accession #: NP_001009613.1
• NCBI GenBank Nucleotide #: NM_001009613.2
• UniProt Accession #: Q5MJ08; Q0ZNK6; Q5W0S6
• Molecular Weight: 11,168 Da
• NCBI Official Full Name: sperm protein associated with the nucleus on the X chromosome N4
• NCBI Official Synonym Full Names: SPANX family, member N4
• NCBI Official Symbol: SPANXN4
• NCBI Official Synonym Symbols: CT11.9
• NCBI Protein Information: sperm protein associated with the nucleus on the X chromosome N4
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome N4
• Protein Family: Sperm protein associated with the nucleus on the X chromosome
• UniProt Gene Name: SPANXN4
• UniProt Synonym Gene Names: SPANX-N4
• UniProt Entry Name: SPXN4_HUMAN

NCBI Description

This gene represents one of several duplicated family members that are located on the X chromosome. This gene family encodes proteins that play a role in spermiogenesis. These proteins represent a specific subgroup of cancer/testis-associated antigens, and they may be candidates for tumor vaccines. This family member belongs to a subgroup of related genes that are present in all primates and rats and mice, and thus, it represents one of the ancestral family members. [provided by RefSeq, Sep 2009]

Uniprot Description

SPANXN4: one of several duplicated family members that are encoded on the X chromosome. This gene family encodes proteins that play a role in spermiogenesis. These proteins represent a specific subgroup of cancer/testis-associated antigens, and they may be candidates for tumor vaccines. This family member belongs to a subgroup of related genes that are present in all primates and rats and mice, and thus, it represents one of the ancestral family members. [provided by RefSeq, Sep 2009]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.3