SOX10 sirna

SOX10 siRNA (Human)

Cat. Num. AAA8241337

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SOX10; SOX10 siRNA (Human); Transcription factor SOX-10; SOX10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SOX10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 466

• Applicable Applications for SOX10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SOX10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SOX10 sirna

siRNA to inhibit SOX10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NM_006941.3
• UniProt Accession #: P56693; Q6FHW7; B4DV62
• Molecular Weight: 31,088 Da
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• Protein Family: Transcription factor
• UniProt Gene Name: SOX10
• UniProt Entry Name: SOX10_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extrinsic to mitochondrial outer membrane; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; protein binding; transcription coactivator activity; chromatin binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; developmental growth; positive regulation of neuroblast proliferation; anatomical structure morphogenesis; positive regulation of gliogenesis; in utero embryonic development; cell maturation; digestive tract morphogenesis; enteric nervous system development; peripheral nervous system development; regulation of transcription from RNA polymerase II promoter; melanocyte differentiation; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural crest cell migration; negative regulation of apoptosis

Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 4c; Waardenburg Syndrome, Type 2e