SORBS3 sirna

SORBS3 siRNA (Human)

Cat. Num. AAA8232547

• Gene Names: SORBS3; SCAM1; SH3D4; SCAM-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SORBS3; SORBS3 siRNA (Human); SCAM1; Vinexin; SH3-containing adapter molecule 1; SCAM-1; Sorbin and SH3 domain-containing protein 3; SORBS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SORBS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for SORBS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SORBS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SORBS3 sirna

siRNA to inhibit SORBS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 65301112
• NCBI GeneID: 10174
• NCBI Accession #: NP_001018003.1
• NCBI GenBank Nucleotide #: NM_001018003.2
• UniProt Accession #: O60504; Q5BJE4; Q6NX54; Q96FY4; Q9UQE4
• Molecular Weight: 36,742 Da
• NCBI Official Full Name: vinexin isoform 2
• NCBI Official Synonym Full Names: sorbin and SH3 domain containing 3
• NCBI Official Symbol: SORBS3
• NCBI Official Synonym Symbols: SCAM1; SH3D4; SCAM-1
• NCBI Protein Information: vinexin
• UniProt Protein Name: Vinexin
• Protein Family: Vinexin
• UniProt Gene Name: SORBS3
• UniProt Synonym Gene Names: SCAM1; SCAM-1
• UniProt Entry Name: VINEX_HUMAN

NCBI Description

This gene encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization, cell adhesion and migration, signaling, and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

vinexin: an adapter protein that contains three SH3 domains and is involved in the reorganization of actin cytoskeleton. Two alternatively spliced isoforms have been described. Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation by using its third SH3 domain. Both isoforms localize at focal adhesion and cell-cell adhesion sites; vinexin beta was also found in the nucleus. Interacts with DLG5 through its third SH3 domain. Interacts with vinculin by the first two SH3 domains and the proline rich region of vinculin. Binds to SOS (guanine nucleotide exchange factor of RAS and RAC) through its third SH3 domain. The formation of this complex is down-regulated by phosphorylation of SOS. Interacts with SAFB2.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: focal adhesion; cytoskeleton; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton; transcription factor binding; vinculin binding

Biological Process: positive regulation of MAPKKK cascade; muscle contraction; positive regulation of stress fiber formation; actin filament organization; positive regulation of cytoskeleton organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; cell adhesion; cell-substrate adhesion